Overview of Shiitake Genome Database

Lentinula edodes, one of the most popular edible mushroom species with high nutrition and special flavor, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with strong wood degradation ability, L. edodes has the potential application in bioenergy production. However, genes, pathways and mechanisms regarding this function are still unknown. Here, we report its 41.8-Mb genome, encoding 14,889 predicted genes, obtained via next-generation sequencing technology. L. edodes’ phylogenies and wood-degrading machinery were investigated by a comparative analysis with the model species of fungi. A total of 93 genes predicted to encode transcription factors and 41 genes encoding candidate lignocellulases are up-regulated when L. edodes mycelia were cultured in sawdust medium versus CYM medium.These differentially expressed genes may play important roles in lignocellulose degradation, and the research of these genes may be beneficial to bioenergy industry, environmental improvement and mushroom production. The overall results will facilitate a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L. edodes cultivation.

Now in this ShiitakeGDB, you can use the Nuclear genome GBrowse2 and blast tools or download genome data. Many other functions of this database are under developing.

Citations:

  1. Chen, L. F., Gong, Y. H., ... & Bian, Y, B. (2016). Genome sequence of the edible cultivated mushroom Lentinula edodes (shiitake) reveals insights into Lignocellulose degradation. Plos One.
  2. Tang, L. H., Jian, H. H., Song, C. Y., Bao, D. P., Shang, X. D., Wu, D. Q., ... & Zhang, X. H. (2013). Transcriptome analysis of candidate genes and signaling pathways associated with light-induced brown film formation in Lentinula edodes. Applied microbiology and biotechnology, 97(11), 4977-4989.
Last updated: March 28th 2016
Copyright: Institue of Applied Mycology, Huazhong Agricultral University, Wuhan, Hubei, China.
For questions and comments, please send email to chenllianfu@foxmail.com or bianyb.123@163.com.